National Institute of Plant Genome Research
Digital India     
 
    Dr. Ashutosh Pandey
    Staff Scientist III
    Tel: 91-11-26741612,14,17 Ext.- 236
    Direct +91-11-26735236
    Fax: 91-11-26741658
    Email: ashutosh@nipgr.ac.in, ashutosh_biotech@yahoo.co.in
 Research Area

Plant molecular biology, bioinformatics and phytochemistry to study the nutritional traits in crop plants (Banana and chickpea) 

 Career:
Staff Scientist III (2021-present): National Institute of Plant Genome Research, New Delhi.
Staff Scientist II (2017-2020): National Institute of Plant Genome Research, New Delhi.
Alexander von Humboldt Fellow (2016-2017): CeBiTec, University of Bielefeld, Germany.
Project Scientist (2013-2016): National Agri-Food Biotechnology Institute, Mohali, India.
 Awards and Honors:
Fellow: Alexander von Humboldt Foundation, Germany (2019).
Member: Indian National Young Academy of Science (INYAS), New Delhi (2018).  
INSA Medal for Young Scientist: Indian National Science Academy (INSA), New Delhi (2017).
 Research Interest:

Understanding regulation of plant specialized metabolism and metabolic engineering of crop plants

Several plant specialized metabolites are of immense human interest owing to their health benefits. Certain secondary metabolites, for example flavonoids can be taken as neutraceuticals in diet. However, several commonly consumed foods are deficient in the content of these health beneficial compounds. It is therefore desirable to improve the content of such compounds in crop plants through application of genetic engineering.
My broad research focus is to understand molecular basis of plant specialized metabolism and to use developed knowledge in metabolic engineering of plants for enhancement of the content of health beneficial plant specialized metabolites. Being crop for major world population including India, banana and chickpea are an attractive target for metabolic engineering of health beneficial plant specialized metabolites. Release of genome sequence of both the crops have provided ample resource to understand aspects of specialized metabolism at molecular level. The major objective is to identify regulatory proteins which regulate the biosynthesis of different classes of flavonoids such as flavonol, anthocyanin and proanthocyanidnes. The identification of such transcription factors will be useful in developing transgenic value added banana enriched in health beneficial flavonoids. To achieve this objective, I employ tools of molecular biology, bioinformatics and phytochemistry. With this, my lab aims to develop value added crops enriched with health beneficial specialized metabolites.

Development of Value-added biomolecules through transformation of abundant plant biomass: (Waste to value)

Value added biomolecules has always remained on top priority for generation of bio-value in the abundant plant biomass, which otherwise turn into agro-industrial waste or by product in excessive quantity. There is huge gap in developing a product involving appropriate delivery system from biomolecule enriched plant biomass which is otherwise a waste. This requires innovations in development of improved biocatalysts, engineering of cell factories with modified metabolic efficiency, and nano-biotechnological tools to bioprocess the plentiful plant biomass. It is envisaged that product through this innovation could be of immense societal benefit especially for healthcare. Banana peel (BP) comprises about 30–40% (w/w) of fresh fruit biomass. The fruit processing, packing, distribution and consumption, generates huge amount of waste or residual biomass. The residual wastes of this huge quantity, not only pose serious environmental risks, but also represent an immense loss of nutrients and bioactive compounds therein. We are exploring biotechnological solutions to utilize the abundant BP biomass for production of high-value biomolecules.

 
 Group Member
Ruchika Rajput Ph.D. Student
Jogindra Naik Ph.D. Student
Samiksha Saxena Ph.D. Student
Samar Singh Ph.D. Student
Biswaranjan Rout Project Associate
  
 Selected Publications
Tiwari M, Trivedi PK, Pandey A (2020) Harnessing gene editing potential of CRISPR-Cas protein for improving agronomic traits in staple crops. Food and Energy SecurityDOI:10.1002/fes3.258
Chatterjee A, Paul A, Unnati GH, Ruchika Biswas T, Kar T, Basak S, Mishra N, Pandey A*, Srivastava AP* (2020)1 MAPK cascade gene family in Camellia sinensis: In-silico identification, expression profiles and regulatory network analysis. BMC Genomics 21:613 
Pucker B, Pandey A, Weisshaar B, Stracke R (2020) The R2R3-MYB gene family in banana (Musa acuminata): genome-wide identification, classification and expression patterns. Plos One 15(10): e0239275.
Praveen A, Pandey A, Gupta M (2020) Protective role of nitric oxide on nitrogen-thiol metabolism and amino acids profiling during arsenic exposure in Oryza sativa L. Ecotoxicology 29, 825–836.
Kaur N, Alok A, Shivani, Kumar P, Kaur N, Awasthi P, Chaturvedi S, Pandey P, Pandey A, Pandey AK, Tiwari S. (2020) CRISPR/Cas9 directed editing of lycopene epsilon-cyclase modulates metabolic flux for β-carotene biosynthesis in banana fruit. Metabolic Engineering. 59:76-86. doi: 10.1016/j.ymben.2020.01.008.
Lakhwani D, Sanchita, Pandey A, Sharma D, Asif MH, Trivedi PK. (2020) Novel microRNAs regulating ripening‑associated processes in banana fruit. Plant Growth Regulation 90:223–235
Dhar YV, Lakhwani D, Pandey A, Singh S., Trivedi PK., Asif MH. (2019) Genome-wide identification and interactome analysis of members of two-component system in Banana. BMC Genomics. 20(1):674.
Agarwal P, Pathak S, Kumar RS, Dhar YV, Pandey A, Shukla S, Trivedi PK. (2019) 3'O-Methyltransferase, Ps3'OMT, from opium poppy: involvement in papaverine biosynthesis. Plant Cell Reports. 38(10):1235-1248.
Praveen A, Pandey A, Gupta M. (2019) Nitric oxide alters nitrogen metabolism and PIN gene expressions by playing protective role in arsenic challenged Brassica juncea L. Ecotoxicology and Environmental Safety 176:95-107.
Bhatia C, Pandey A, Gaddam SR, Hoecker U & Trivedi PK. (2018) Low Temperature-Enhanced Flavonol Synthesis Requires Light-Associated Regulatory Components in Arabidopsis thaliana.Plant & Cell Physiology 59(10): 2099-2112.
Kaur N, Pandey A, Sharma S, Kumar P, Kesarwani AK, Mantri SS,Awasthi P and Tiwari S (2017) Regulation of Banana Phytoene Synthase (MaPSY) expression, characterization and their modulation under various abiotic stress conditions. Frontiers in Plant Science. 8: 462 doi. 10.3389/fpls.2017.00462.
Agan F, Alok A, Kumar J, Thakur N, Pandey A, Pandey AK, Upadhyay SK, & Tiwari S (2016) Characterization and expression analysis of phytoene synthase from bread wheat (Triticum aestivum L.). PloS ONE DOI: 10.1371/journal.pone.0162443.
Pandey A*, Alok A, Lakhwani D, Singh J, Asif MH, Trivedi PK* (2016) Genome-wide Expression Analysis and Metabolite Profiling Elucidate Transcriptional Regulation of Flavonoid Biosynthesis and Modulation under Abiotic Stresses in Banana. Scientific Reports  6:31361. doi: 10.1038/srep31361.
Sharma D, Tiwari M, Pandey A, Bhatia C, Sharma A and Trivedi PK (2016) MicroRNA858 is a potential regulator of phenylpropanoid pathway and plant development in Arabidopsis. Plant Physiology: 17, 944-959.
Pandey A*, Misra P, Alok A, Kaur N, Sharma S, Lakhwani D, Asif MH, Tiwari S and Trivedi PK*. Genome wide identification and expression analysis of Homeodomain leucine zipper subfamily IV (HDZ IV) gene family from Musa accuminata. Frontiers in Plant Science: doi: 10.3389/fpls.2016.00020.
Choudhary D, Pandey A, Adhikary S, Ahmad N, Bhatia C, Bhambhani S, Trivedi PK, Trivedi R. Genetically engineered flavonol enriched tomato fruit modulates chondrogenesis to increase bone length in growing animals. Scientific Reports  6,21668.
Goel R, Pandey A, Trivedi PK, and Asif, MH Genome-wide analysis of the Musa WRKY gene family: evolution and differential expression during development and stress. Frontiers in Plant Science: 7:299.
Lakhwani D, Pandey A, Dhar YV, Bag SK., Trivedi PK, Asif MH Genome-wide analysis of the AP2/ERF family in Musa species reveals divergence and neofunctionalisation during evolution. Scientific Reports. 5, 18878.
Pandey A, Misra P, Choudhary D, Yadav R, Goel R, Bhambhani S, Sanyal I, Trivedi R, and Trivedi PK (2015). AtMYB12 expression in tomato leads to large scale differential modulation in transcriptome and flavonoid content in leaf and fruit tissues. Scientific Reports  5, 12412.
Pandey A, Misra P, and Trivedi PK (2015). Constitutive expression of Arabidopsis MYB transcription factor, AtMYB11, in tobacco modulates flavonoid biosynthesis in favor of flavonol accumulation. Plant Cell Reports, 34, 1515-1528.
Pandey A, Misra P, Bhambhani, S and Trivedi PK (2014) Expression of Arabidopsis MYB transcription factor, AtMYB111, in tobacco requires light to modulate flavonol content. Scientific Reports  21, 5018.
Pandey A, Misra P, Khan MP, Swarnker G, Tewari MC, Bhambhani S, Trivedi R, Chattopadhyay N and Trivedi PK (2014) Coexpression of Arabidopsis transcription factor, AtMYB12, and soybean isoflavone synthase, GmIFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. Plant Biotechnology Journal 12, 69-80.
Pandey A, Misra P, Chandrashekhar K and Trivedi PK (2012) Development of AtMYB12-expressing transgenic tobacco callus culture for production of rutin with biopesticidal potential. Plant Cell Reports, 31, 1867–1876.
Misra P, Pandey A, Tewari M, Chandrashekhar K, Siddhu OP, Asif MH, Chakrabarty D, Singh PK, Nath P, Trivedi PK and Tuli R (2010) Modulation of transcriptome and metabolome by AtMYB12 transcription factor leads to insect tolerance. Plant Physiology, 152, 2258-2268.
Misra P, Pandey A, Tewari SK, Nath P and Trivedi PK (2010) Characterization of isoflavone synthase gene from Psoralea corylifolia: a medicinal plant. Plant Cell Reports, 29, 747–755.
Book chapters:
Ruchika, Naik J, Pandey A (2019) Synthetic Metabolism and Its Significance in Agriculture. In: Current Developments in Biotechnology and Bioengineering Synthetic Biology, Cell Engineering and Bioprocessing Technologies pp 365-391. Elsevier. ISBN: 9780444640864
Singh SP, Upadhyay SK, Pandey A, Kumar S (2019) Molecular Approaches in Plant Biology and Environmental Challenges Editors: Singh SP, Upadhyay SK, Pandey A, Kumar S (Eds.) pp 1-5. Springer-Verlag Ltd. Singapore: ISBN 978-981-15-0690-1
Kaur N, Shivani, Pandey A, and Tiwari S. (2016) Provitamin A enrichment for tackling malnutrition. In: Mohandas S, Ravishankar KV (eds) Banana: Genomics and transgenic approaches for genetic improvement. Springer-Verlag Ltd. Singapore: pp 277–300. DOI 10.1007/978-981-10-1585-4_19
Book edited:
Molecular Approaches in Plant Biology and Environmental Challenges Editors: Singh SP, Upadhyay SK, Pandey A, Kumar S (Eds.) Springer-Verlag Ltd. Singapore: ISBN 978-981-15-0690-1