National Institute of Plant Genome Research
Digital India     
 
    Dr. Hasthi Ram
    Staff Scientist II,
    National Institute of Plant Genome Research (NIPGR), New Delhi
    Tel:  91-11-26735176
    Fax:  91-11-26741658
    E-mail: hasthi.ram@nipgr.ac.in, hrmundel84@gmail.com
 Research Area
Crop genetics and genomics
 Career:
Staff Scientist II (2020- Present): National Institute of Plant Genome Research (NIPGR), New Delhi, India
DST-INSPIRE Faculty (2017-2020): National Agri-Food Biotechnology Institute (NABI), Mohali
Postdoctoral Fellow (2012-2016): European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
Ph.D. (2007-2012): National Institute of Plant Genome Research (NIPGR), New Delhi
 Awards & Honors:
Young Scientist Platinum Jubilee Award from the National Academy of Sciences, India (NASI) (2019).
DST-INSPIRE Faculty award by DST, Govt. of India (2017).
NET- JRF by CSIR, Govt. of India (2007).
GATE (Graduate Aptitude Test in Engineering) by MHRD, Govt. of India (2007).
 Research Interests

During post-harvest processing (polishing/milling) of brown rice, bran layer consisting of aleurone layer, pericarp and embryo is removed to produce white rice. Bran is nutritionally superior as it has major reservoirs of various minerals, vitamins, essential mineral oils and other bioactive compounds, whereas white rice portion is nutritionally inferior as it mainly contains starchy endosperm. Irony is that nutritionally inferior white rice is a major staple food for almost half of the country, whereas almost 2/3rd of nutrient rich bran fraction is often get wasted as inedible due to instability of the bran. Thus both the products of the rice post-harvest process, the white rice and the bran, have their own inherent problems. We aim to overcome both these problems using biotechnological approaches. This will help to increase nutritional value of the white rice and increase the stability of bran. Towards this end, using high-resolution tissue-type specific transcriptomics approach we have prepared a gene expression atlas of developing rice grain and identified cell/tissue-type specific genes. From this very highly-resolved gene expression dataset, we have identified certain genes which might be potentially involved in micronutrient accumulation, particularly iron and vitamin A, in the grain, and we are analyzing their role in nutritional enhancement of the white rice. For increasing the stability of bran we are looking at role of various hydrolytic enzymes such as lipases, estrases, etc., which have very tissue-type specific expression in our transcriptomics dataset. We are using various molecular biology tools such as cell-type specific transcriptomics, in-situ hybridisation, confocal imaging, CRISPR-Cas9 system, expression in heterologous system and plant tissue culture approaches for our studies.

We are a new group and welcome potential PDFs, PhDs, Fellows, Trainees, etc. to contact the PI directly.

 Publications

Soni P, Shivhare R, Kaur A, Bansal S, Sonah H, Deshmukh R, Giri J, Lata C, Ram H* (2021). Reference gene identification for gene expression analysis in rice under different metal stress. Journal of Biotechnology (In press) DOI: 10.1016/j.jbiotec.2021.03.019 (*Corresponding author, IF=3.2)
Ram H*, Sardar S, Gandass N (2021) Vacuolar Iron Transporter-(Like) Proteins: Regulators of Cellular Iron Accumulation in Plants. Physiologia Plantarum (https://doi.org/10.1111/ppl.13363) (*Corresponding author) (IF=4.1)
Shivaraj SM, Sharma Y, Chaudhary J, Rajora N, Sharma S, Thakral V, Ram H, Sonah H, Singla-Pareek SL, Sharma TR, Deshmukh R (2021) Dynamic role of aquaporin transport system under drought stress in plants. 484, 104367, Environmental and Experimental Botany. (IF=4.0)
Ram H*, Singh A, Katoch M, Kaur R, Sardar S, Palia S, Satyam R, Sonah H, Deshmukh R, Pandey A, Gupta I, Sharma TR (2020) . Dissecting the nutrient partitioning mechanism in rice grain using spatially resolved gene expression profiling. Journal of Experimental Botany, eraa536. (*Corresponding author) https://doi.org/10.1093/jxb/eraa536
Ram H, Sahadevan S, Caggiano MP, Yu X, Ohno C, Heisler MG. (2020). An integrated analysis of cell-type specific gene expression reveals targets of REVOLUTA and KANADI1 in the Arabidopsis shoot apical meristem. PLoS Genetics 16(4): e1008661. 10.1371/journal.pgen.1008661
Ram H*, Gandass N, Sharma A, Singh A, Sonah H, Deshmukh R, Pandey AK, Sharma TR (2020). Spatio-temporal distribution of micronutrients in rice grains and its regulation. Critical Reviews in Biotechnology. 40(4):490-507, (*Corresponding author). https://doi.org/10.1080/07388551.2020.1742647
Sharma S, Kaur G, Kumar A, Ram H, Kaur J, Pandey AK. (2020) Gene Expression Pattern of Vacuolar-Iron Transporter-Like (VTL) Genes in Hexaploid Wheat during Metal Stress. Plants 9(2), 220.
Ram H*, Kaur A, Gandass N, Singh S, Sonah H, Deshmukh R, Sharma TR (2019). Molecular characterization and expression dynamics of MTP genes under various spatio-temporal stages and metal stress conditions in rice. PLoS One 14(5): e0217360. (*Corresponding author)
Ram H*, Soni P, Salvi P, Gandass N, Sharma A, Kaur A, Sharma TR* (2019). Insertional Mutagenesis Approaches And Their Use in Rice for Functional Genomics. Plants, 8(9), 310. (*Corresponding author)
Chakraborty M, Gangappa SN, Maurya JP, Sethi V, Srivastava A, Singh A, Dutta S, Gupta N, Sengupta M, Ojha M, Ram H, Chattopadhyay S (2019). Functional Interrelation of MYC2 and HY5 Plays an Important Role in Arabidopsis Seedling Development. Plant Journal, https://doi.org/10.1111/tpj.14381
Ram H, Singh BP, Katara J, Kumar P, Jaiswal R, Geetika, Deshmukh R, Sonah H (2019). A Genome-wide Resource of Intron Spanning Primers Compatible for Quantitative PCR and Intron Length Polymorphism in Rice. Indian Journal of Genetics and Plant Breeding. 79(2).
Caggiano MP, Yu X, Bhatia N, Larsson A, Ram H, Ohno CK, et al. (2017) Cell type boundaries organize plant development. eLife. 6. DOI: 10.7554/eLife.27421 (IF=7.6)
Giri MK, Banday ZZ, Singh N, Singh V, Ram H, Singh D, Chattopadhyay S, Nandi AK (2017)  GBF1 differentially regulates CAT2 and PAD4 transcription to promote pathogen defense in Arabidopsis thaliana. The Plant Journal. 91(5): 802–815 https://doi.org/10.1111/tpj.13608 (IF=5.8)
Merelo P, Ram H, Pia Caggiano M, Ohno C, Ott F, Straub D, Graeff M, Cho SK, Yang SW, Wenkel S, et al. (2016) Regulation of MIR165/166 by Class II and Class III homeodomain leucine zipper proteins establishes leaf polarity. Proceedings of the National Academy of Sciences (PNAS), USA, 2016, 113 (42), 11973-11978. https://doi.org/10.1073/pnas.1516110113 (IF=9.5)
Ram H, Jain M, Singh A, Chattopadhyay S (2016) Functional Relationship of GBF1 with HY5 and HYH in Genome-Wide Gene Expression in Arabidopsis. Plant Molecular Biology Reporter. 34(1): 211-220. DOI: 10.1007/s11105-015-0910-x (IF=2.0)
Ram H, Priya P, Jain M, Chattopadhyay S  (2014) Genome-wide DNA binding of GBF1 is modulated by its heterodimerizing protein partners, HY5 and HYH. Molecular Plant. 7(2): 448-451. DOI: http://dx.doi.org/10.1093/mp/sst143 (IF=12.2)
Gangappa SN, Srivastava AK, Maurya JP, Ram H, Chattopadhyay S  (2014) Z-box binding transcription factors (ZBFs): a new class of transcription factors in Arabidopsis seedling development. Molecular Plant. 6(6): 1758-1768 DOI: http://dx.doi.org/10.1093/mp/sst140 (IF=12.2)
Ram H, Chattopadhyay S (2013) Molecular interaction of bZIP domains of GBF1, HY5 and HYH in Arabidopsis seedling development.  Plant Signaling & Behavior. 8(1): e22703 DOI:  https://doi.org/10.4161/psb.22703 (IF~1.0)
Ram H#, Singh A#, Abbas N, Chattopadhyay S (2012) Molecular interactions of GBF1 with HY5 and HYH proteins during light-mediated seedling development in Arabidopsis thaliana. J Biol Chem. 287(31): 25995-6009 (#Equal contribution). DOI:10.1074/jbc.M111.333906 (IF=4.0)
Mallappa C, Singh A, Ram H, and Sudip Chattopadhyay (2008) GBF1, a transcription factor of blue light signaling in Arabidopsis, is degraded in the dark by a proteasome-mediated pathway independent of COP1 and SPA1. J Biol Chem. 283: 35772-35782  DOI: 10.1074/jbc.M803437200 (IF=4.0)